KMID : 0381120200420080855
|
|
Genes and Genomics 2020 Volume.42 No. 8 p.855 ~ p.867
|
|
Two way network construction and analysis of mRNA, miRNA and lncRNA reveals critical regulators and regulatory modules in cardiovascular diseases
|
|
Charles Sona
Natarajan Jeyakumar
|
|
Abstract
|
|
|
Background: Cardiovascular diseases contribute to the leading cause of deaths (31%) in the world population.
Objective: The objective of this study is to compile non-coding RNA-gene interaction into a core regulatory network whose dysregulation might play an important role in disease progression.
Method: We applied a structured approach to reconstruct the interaction network of lncRNAs, miRNAs and genes involved in cardiovascular diseases. For network construction, we used ¡®diseasome to interactome¡¯ and ¡®interactome to diseasome¡¯ approaches and developed two regulatory networks in heart disorders. In diseasome to interactome approach, starting from a disease-centric network we, expanded the data into an interaction network. However in interactome to diseasome, we used a set of guide genes, miRNAs and lncRNAs to arrive at the common diseases. The disease-centric network in combination with the interaction network will shed light on the interconnected components in a huge diseasome network implicated in heart disorders and manifested through small sub-networks while progressing. Using the above networks we created a sub-networks consisting only of hub genes, miRNAs and lncRNAs on both approaches. The dysregulation of any one of the hubs can lead to a disease condition.
Results: The top ranking hubs common in both the sub-networks were found to be VEGFA, MALAT1, HOTAIR, H19 and hsa-miR-15a. Our network based study reveals an entanglement of regulatory sub-network of miRNAs, lncRNAs and genes in multiple conditions.
Conclusion: The identification of hubs in the core triple node network of elements in disease development and progression demonstrates a promising role for network based approaches in targeting critical molecules for drug development.
|
|
KEYWORD
|
|
lncRNAs, miRNAs, Gene regulatory network, Cardiovascular diseases, Diseasome, Interactome
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|